CALL FOR PAPERS Proteomic and Metabolomic Approaches to Cell Physiology and Pathophysiology Proteasome inhibition in skeletal muscle cells unmasks metabolic derangements in type 2 diabetes
نویسندگان
چکیده
Lubna Al-Khalili, Thais de Castro Barbosa, Jörgen Östling, Julie Massart, Pablo Garrido Cuesta, Megan E. Osler, Mutsumi Katayama, Ann-Christin Nyström, Jan Oscarsson, and Juleen R. Zierath Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden; Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; AstraZeneca Research and Development, Mölndal, Sweden; and Department of Functional Biology, Physiology Area, University of Oviedo, Oviedo, Spain
منابع مشابه
Proteasome inhibition in skeletal muscle cells unmasks metabolic derangements in type 2 diabetes.
Two-dimensional difference gel electrophoresis (2-D DIGE)-based proteome analysis has revealed intrinsic insulin resistance in myotubes derived from type 2 diabetic patients. Using 2-D DIGE-based proteome analysis, we identified a subset of insulin-resistant proteins involved in protein turnover in skeletal muscle of type 2 diabetic patients, suggesting aberrant regulation of the protein homeos...
متن کاملCALL FOR PAPERS Proteomic and Metabolomic Approaches to Cell Physiology and Pathophysiology Understanding the acetylome: translating targeted proteomics into meaningful physiology
Philp A, Rowland T, Perez-Schindler J, Schenk S. Understanding the acetylome: translating targeted proteomics into meaningful physiology. Am J Physiol Cell Physiol 307: C763–C773, 2014. First published September 3, 2014; doi:10.1152/ajpcell.00399.2013.—It is well established that exercise elicits a finely tuned adaptive response in skeletal muscle, with contraction frequency, duration, and reco...
متن کاملThe Effect of Resistance Training and Endothelial Stem Cell Injection on Skeletal Muscle Oxidant and Antioxidant Status in STZ-Induced Diabetic Male Rats
Background: Because insulin therapy cannot properly control the progression of diabetes and its complications, other alternative therapies may be desirable. The aim of this study was to investigate the effect of resistance training and endothelial stem cell injection on skeletal muscle oxidant and antioxidant status in STZ-induced diabetic male rats. Method: In this experimental study, 36 male...
متن کاملSimultaneous Effect of Resistance Training and Stem Cell Injection on Blood Glucose Levels and Bax and Bcl2 Protein Expression from Markers of Skeletal Muscle Apoptosis in STZ-Induced Diabetic Male Rats
Background: Type 1 diabetes is a disorder caused by autoimmune destruction of pancreatic insulin-producing cells. This induction of autoimmunity may be due to genetic and environmental factors. Bax and Bcl2 proteins play an important role in the process of apoptosis. Methods: In this study, 30 male Wistar rats weighting approximately 200±20gr were randomly selected from available rats in lab ...
متن کاملIsolation and optimization of mice skeletal muscle satellite cells using preplating method and culture media substitution
Introduction: Satellite cells are known as the main regenerative cell type in skeletal muscles. Our study established a modified digestion and preplating method for the isolation of slow or weak adherent cells for the enrichment of satellite cells. Low-survival rate of these primary stem cells prompted us to address whether cell culture medium substitution might change cell viability status. M...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2014